The purpose of this study is to determine if new medical devices can detect the level of hemoglobin (the red pigment in the blood that carries oxygen) in the human body. The devices shine light through the skin, similar to a pulse oximeter, or measure the electrical properties of tissues, such as a calf muscle.
On the day of the experiment, you will spend between 2.5 and 8 hours doing the test procedures (the time required depends on the number of units of blood removed and re-infused). 1 to 4 pints of blood are withdrawn and are replaced with other fluid. This blood donation procedure is called hemodilution, which is defined as the replacement of blood with an equal amount of fluid. The blood that has been removed will be returned after processing to remove excess fluid. One pint of the blood that was removed may not be returned to you.
Pulse oximeters and hemoglobin measurement devices will be attached to your fingers, forehead and or calf. Electrocardiogram pads will be placed on the skin of your chest.
You will have catheters (small plastic tubes) placed in vein(s) in one or both arms and an artery of one of your wrists. Before inserting the catheter into your wrist artery, we will check blood flow in your hand by occluding the wrist artery by finger pressure while you make a fist. One to 4 pints of blood (the amount will depend on your weight and starting hemoglobin concentration) will be withdrawn while 5% Human Albumin, a solution of a common blood plasma protein, will be returned through a vein to keep the total amount of fluid in your body at a normal level. All but one of the units of blood that is withdrawn will be processed by the UCSF Blood Bank so that your red blood cells from those units can be returned. The plasma will not be returned to you during the transfusion.
Up to 30 small blood samples will be taken during the study to measure oxygen carbon dioxide, calcium, and other tests (the blood will be taken from the catheter placed in my artery). The total amount of blood taken for these tests is about 2 ounces.
You may be asked to breathe, via a mouthpiece, an air mixture containing reduced amounts of oxygen, as would occur at high altitude. This will be done for just a few minutes.
The physician will be immediately available for emergencies. The subject will be allowed to return home accompanied by a friend or family member. Subjects will be called 48-72 hrs after the study and if they have any symptoms or concerns they will speak with a study MD who may request they return to UCSF to see them if any additional treatment is needed.
Study volunteers may choose to stop the study at any point.
Additional Exclusion Criteria
History of migraine headaches.
Anyone weighing over 80kg (176 lbs)
Any medical condition that the study doctors think will put our study subject’s safety at risk.
Hemodilution Study Consent
It is necessary to sign a consent for the study. You can download the Hemodilution Study Consent Form ahead of time, which should be read in advance so that we can take time to answer any questions.